Journal: Methods in Molecular Biology

Article Title: Allergy

doi: 10.1007/978-1-4939-9591-2

Figure Lengend Snippet: Fig. 1 Different types of immunoelectrophoresis of cat hair extract (Fel d) using a polyclonal rabbit antibody raised against the same extract (a Fel d). (a) CIE of Fel d extract, (b) FRIE following a purification (immunoab- sorption) of the major allergen Fel d 1, (c) CIE of purified Fel d 1 against a Fel d, (d) CLIE with Fel d 1 in inter- mediate gel for identification of the allergen, (e) TCIE with Fel d extract and Fel d 1 showing a double peak, (f) CIIE with monospecific Fel d 1 in intermediate gel

Article Snippet: Detection: Biotinylated goat anti-human MIG polyclonal (R&D Systems #BAF392 or PeproTech).

Techniques: Immunoelectrophoresis, Purification

Journal: The Journal of Clinical Investigation

Article Title: Herpes simplex virus-1 evasion of CD8 + T cell accumulation contributes to viral encephalitis

doi: 10.1172/JCI92931

Figure Lengend Snippet: Five-week-old female ICR mice were mock-infected or infected with 1 × 106 PFU UL13R or UL13KM per eye. At 7 days after infection, brain stem samples were processed, and the amounts of Cxcl9 (A), Cxcl10 (B), Cxcl11 (C), and Il-6 (D) mRNA were analyzed by quantitative RT-PCR. The results from 2 independent experiments (1 with 4 mice and 1 with 5 mice) were combined. Each data point is the relative amount of each mRNA in the brain stem of one mouse. The statistical significance values were analyzed by the Mann-Whitney U test.

Article Snippet: Goat polyclonal antibody to CXCL9 (AF-492-NA; R&D Systems), mouse monoclonal antibody to β-actin (AC15; Sigma-Aldrich), donkey anti-goat IgG-HRP (sc-2020; Santa Cruz Biotechnology Inc.), and sheep anti-mouse IgG, HRP-linked F(ab′) 2 fragment (NA9310; GE) were used for immunoblotting.

Techniques: Infection, Quantitative RT-PCR, MANN-WHITNEY

Journal: The Journal of Clinical Investigation

Article Title: Herpes simplex virus-1 evasion of CD8 + T cell accumulation contributes to viral encephalitis

doi: 10.1172/JCI92931

Figure Lengend Snippet: (A–G) Five-week-old female ICR mice were ocularly infected with 1 × 106 PFU UL13R per eye. At 5 days after infection, CXCL9 or PBS was injected into the brain stems of the infected mice. At 7 days after infection, brain stem (A and E) and submandibular lymph node (B and F) samples were processed and analyzed for CD8+ T (CD8+, CD3+, and CD45+) (A and B) or CD4+ T (CD4+, CD3+, and CD45+) (E and F) cell content by flow cytometry. At 7 days after infection, CD8+ T cells purified from brain stem (C) and submandibular lymph node samples (D) were assayed for IFN-γ–producing cell content by ELISPOT assays. At 5 and 7 days after infection, viral titers in the brains of infected mice were assayed (G). The results from 3 independent experiments (each with 4 mice) were combined. Each data point is the number of each type of cells in each tissue of one mouse (A–F) or the virus titer in the brain of one mouse (G). (H) Survival was monitored daily for 21 days. The results from 4 independent experiments (1 with 5 mice and 3 with 6 mice for the CXCL9 injection experiments; and 1 with 3 mice, 1 with 4 mice, and 2 with 5 mice for the PBS injection experiments) were combined. The statistical significance values were analyzed by the Mann-Whitney U test (A–G) or the log-rank test (H).

Article Snippet: Goat polyclonal antibody to CXCL9 (AF-492-NA; R&D Systems), mouse monoclonal antibody to β-actin (AC15; Sigma-Aldrich), donkey anti-goat IgG-HRP (sc-2020; Santa Cruz Biotechnology Inc.), and sheep anti-mouse IgG, HRP-linked F(ab′) 2 fragment (NA9310; GE) were used for immunoblotting.

Techniques: Infection, Injection, Flow Cytometry, Purification, Enzyme-linked Immunospot, Virus, MANN-WHITNEY

Journal: The Journal of Clinical Investigation

Article Title: Herpes simplex virus-1 evasion of CD8 + T cell accumulation contributes to viral encephalitis

doi: 10.1172/JCI92931

Figure Lengend Snippet: Five-week-old female ICR mice depleted of CD8+ T cells were infected with 1 × 106 PFU UL13R per eye. At 5 days after infection, CXCL9 or PBS was injected into the brain stems of the infected mice. (A) At 5 and 7 days after infection, viral titers in the brains of infected mice were assayed. The results from 2 independent experiments (each with 4 mice) were combined. Each data point is the virus titer in the brain of one mouse. (B) Survival was monitored daily for 21 days. The results from 4 independent experiments (2 with 6 mice and 2 with 3 mice) were combined. The statistical significance values were analyzed by the Mann-Whitney U test (A) or the log-rank test (B).

Article Snippet: Goat polyclonal antibody to CXCL9 (AF-492-NA; R&D Systems), mouse monoclonal antibody to β-actin (AC15; Sigma-Aldrich), donkey anti-goat IgG-HRP (sc-2020; Santa Cruz Biotechnology Inc.), and sheep anti-mouse IgG, HRP-linked F(ab′) 2 fragment (NA9310; GE) were used for immunoblotting.

Techniques: Infection, Injection, Virus, MANN-WHITNEY